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文献专栏

常用荧光物质的激发波长和发射波长

发表时间:2019-09-06 20:42

Excitation and Emission Wavelengths of Fluorescent Molecule Probes.


荧光物质激发波长发射波长
EX nmEX(Sub)EM nm
Alexa Fluor 532532
554
Cy3550
570
DsRed557
579
EtBr300518605
FITC490
525
Gel Green250500530
GFP488
507
mCherry580
610
SYBR Gold495
540
SYBR Green I498
522
SYPRO Red550300630
SYPRO Ruby280450620
TagRFP555
583
Gel Red270510600



荧光物质名称激发波长发射波长
1, 5 IAEDANS336nm482nm
4-Methylumbelliferone360nm449nm
5-Carboxyfluorescein (5-FAM)®492nm518nm
5-Carboxynaphthofluorescein®598nm668nm
5-Carboxytetramethylrhodamine(5-TAMRA)®542nm568nm
5-FAM (5-Carboxyfluorescein)®492nm518nm
5-ROX (carboxy-X-rhodamine)574nm602nm
5-TAMRA (5-Carboxytetramethylrhodamine)542nm568nm
6-Carboxyrhodamine 6G (6-CR-6G)®518nm543nm
6-CR 6G®518nm543nm
6-JOE525nm555nm
7-Amino-4-methylcoumarin®351nm430nm
7-Aminoactinomycin D (7-AAD)®546nm647nm
7-Hydroxy-4-methylcoumarin (pH 9)360nm449nm
AcGFP475nm505nm
Acridine® orange(+DNA)500nm526nm
Acridine® orange(+RNA)460nm650nm
Alexa Fluor® 350346nm442nm
Alexa Fluor® 405401nm422nm
Alexa Fluor® 488498nm520nm
Alexa Fluor® 514517nm539nm
Alexa Fluor® 532532nm553nm
Alexa Fluor® 546556nm573nm
Alexa Fluor® 555553nm568nm
Alexa Fluor® 568 #4433c0 #5544cc578nm603nm
Alexa Fluor® 594590nm617nm
Alexa Fluor® 633632nm647nm
Alexa Fluor® 647649nm666nm
Alexa Fluor® 680680nm702nm
Alexa Fluor® 700696nm719nm
Alexa Fluor® 750752nm779nm
Alexa Fluor® 790782nm805nm
Alexa Fluor® 488/546 FRET495nm573nm
Alexa Fluor® 488/555 FRET495nm568nm
Alexa Fluor® 488/Cy3® FRET495nm570nm
Allophycocyanin (APC)650nm660nm
AMCA/AMCA-X345nm445nm
AmCyan458nm489nm
Aniline Bluer®370nm509nm
ANSr®372nm455nm
AsRed2576nm592nm
Atto 390390nm479nm
Atto 425436nm485nm
Atto 465453nm507nm
Atto 488501nm525nm
Atto 550554nm576nm
Atto 633630nm657nm
Atto 647N644nm669nm
Atto 680680nm700nm
ATTO-TAG™ CBQCA465nm560nm
ATTO-TAr™ FQ486nm591nm
Auraminer O-Feulgen460nm550nm
Azamir Green492nm505nm
BCECF503nm528nm
BCECF/pH 5.2482nm520nm
BCECF/pH 9.0503nm528nm
BFP (Blue Fluorescent Protein)r382nm448nm
BFP/DsRed2 FRET382nm583nm
BFP/eGFP FRET382nm508nm
BFP/YFP FRET382nm527nm
Biosearch Blue352nm447nm
BOBO™-1 BO-PRO™-1462nm481nm
BOBO™-3 BO-PRO™-3570nm604nm
BODIPY® FL/pH7.2505nm512nm
BODIPY® 530/550533nm550nm
BODIPY® 558/568558nm568nm
BODIPY® 564/570564nm570nm
BODIPY® 581/591582nm590nm
BODIPY® 630/650-X630nm650nm
BODIPY® 650/665-X650nm665nm
BODIPY® FL505nm513nm
BODIPY® TMR542nm574nm
BODIPY® TR-X589nm617nm
BTC401nm529nm
CAL Fluor® Gold 540522nm544nm
CAL Fluor® Orange 560538nm559nm
CAL Fluor® Red 590569nm591nm
CAL Fluor® Red 610590nm610nm
CAL Fluor® Red 635618nm637nm
Calcein494nm517nm
Calcein Blue375nm420nm
Calcein®494nm517nm
Calcium Crimson™590nm615nm
Calcium Green-1™506nm531nm
Calcium Orange™549nm576nm
Calcofluor® White350nm440nm
Carboxy-X-rhodamine (5-ROX)574nm602nm
Cascade Blue®400nm420nm
Cascade Yellow™402nm545nm
CCF2 (GeneBLAze™r)402nm520nm
Cerulean433nm475nm
CFP(Cyan Fluorescent Protein)?434nm477nm
CFP/DsRed2 FRET434nm583nm
CFP/YFP FRET434nm527nm
Chromomycin A3450nm470nm
Citrine514nm529nm
Cl-NERF (low pH)504nm540nm
Coumarin384nm470nm
C-phycocyanin620nm648nm
CPM385nm471nm
CryptoLight CF-2584nm657nm
CryptoLight CF-5566nm597nm
CryptoLight CF-6566nm615nm
CTC Formazan450nm630nm
Cy2™489nm506nm
Cy3.5™581nm598nm
Cy3™ /Cy5.5™ FRET550nm694nm
Cy3™ /Cy5™ FRET550nm670nm
Cy3™552nm570nm
Cy5.5™675nm694nm
Cy5™649nm670nm
Cy7™743nm767nm
Cycle 3 GFP395nm507nm
Dansyl cadaverine335nm518nm
Dansylchloride380nm475nm
DAPI358nm461nm
Dapoxyl®373nm574nm
Dendra2 Green490nm507nm
Dendra2 Red553nm573nm
Di-4 ANEPPS488nm605nm
Di-8 ANEPPS468nm635nm
Di-8-ANEPPS non-ratiometric530nm620nm
DiA456nm591nm
DiA (4-Di-16-ASP)491nm613nm
DiD (DilC18(5))644nm665nm
DIDS?341nm414nm
DiI (DiIC18(3))549nm565nm
DiO (DiOC18(3))484nm501nm
DiR (DiIC18(7))750nm779nm
DM-NERF (4.5_6.5 pH)510nm536nm
Draq5647nm683nm
Dronpa Green503nm518nm
DsRed2 (Red Fluorescent Protein)558nm583nm
DsRed-Express557nm579nm
DsRed-Monomer556nm586nm
DyLight 350352nm435nm
DyLight 405399nm433nm
DyLight 488492nm517nm
DyLight 549554nm569nm
DyLight 594592nm616nm
DyLight 633623nm645nm
DyLight 649652nm667nm
DyLight 680677nm705nm
DyLight 750751nm772nm
DyLight 800770nm795nm
EBFP380nm440nm
EBFP2385nm448nm
ECFP433nm475nm
EGFP488nm509nm
EGFP/DsRed 2 FRET470nm585nm
EGFP/Rhod-2 FRET488nm571nm
ELF®-97 alcohol345nm530nm
Emerald487nm509nm
EmGFP487nm509nm
EosFP Green506nm516nm
EosFP Red571nm581nm
Eosin524nm544nm
Erythrosin529nm554nm
Ethidium bromide300nm605nm
Ethidium homodimer-1 (EthD-1)528nm617nm
Europium (III) Chloride337nm613nm
EYFP513nm527nm
FAM492nm518nm
Fast Blue365nm420nm
FITC490nm525nm
FITC/Cy3® FRET494nm570nm
FITC/Rhod 2 FRET494nm571nm
FITC/TRITC FRET494nm580nm
FlAsH-CCPFCC511nm530nm
Fluo-3506nm526nm
Fluo-4494nm516nm
Fluorescein (FITC)494nm518nm
Fluoro-Gold (high pH)368nm565nm
Fluoro-Gold (low pH)323nm408nm
Fluoro-Jade475nm525nm
FluorX®494nm519nm
FM™ 1-43479nm598nm
FM™ 4-64515nm740nm
Fura Red™ Fluo-3472nm672nm
Fura Red™436nm637nm
Fura-2335nm505nm
Fura-2/BCECF335nm528nm
Fura-2/Ca2+ - free340nm510nm
Fura-2/Ca2+ - saturated380nm510nm
FusionRed576nm603nm
Gel Green250530
Gel Red270600
GeneBLAzer™ (CCF2)402nm520nm
GFP488nm507nm
GFP (sapphire)395nm508nm
GFP/Rhod 2 FRET488nm571nm
GFP2395nm510nm
HcRed588nm618nm
HEX,SE533nm559nm
HiLyte Fluor™ 488497nm525nm
HiLyte Fluor™ 555550nm566nm
HiLyte Fluor™ 647649nm672nm
HiLyte Fluor™ 680688nm700nm
HiLyte Fluor™ 750750nm782nm
Hoechst 33258352nm461nm
Hoechst 33342 & 33258352nm461nm
HyPer420nm516nm
Indo-1330nm401nm
Indo-1/Ca2+ -free346nm475nm
Indo-1/Ca2+ -saturated330nm401nm
JC-1498nm595nm
JOE520nm548nm
JOJO™-1, JO-PRO™-1529nm545nm
JRed584nm610nm
Kaede Green508nm518nm
Kaede Red572nm580nm
KeimaRed440nm616nm
KikumeGR Green507nm517nm
KikumeGR Red583nm593nm
KillerRed585nm610nm
Kusabira Orange (mKO)548nm549nm
Li-Cor IRDye® 680LT674nm693nm
Li-Cor IRDye® 800CW778nm794nm
Lissamine rhodamine B570nm590nm
LOLO™-1, LO-PRO™-1565nm579nm
Lucifer Yellow428nm536nm
LysoSensor™ Blue (pH 5)374nm424nm
LysoSensor™ Green (pH 5)442nm505nm
LysoSensor™ Yellow/Blue (pH 4.2)384nm540nm
LysoTracker™ Blue/MeOH373nm425nm
LysoTracker™ Yellow HCK-123486nm563nm
LysoTracker™ Green504nm511nm
LysoTracker™ Red577nm592nm
LysoTracker™ Yellow465nm535nm
mAG (Azami?Green)492nm505nm
Mag-Fura-2330nm491nm
Mag-Indo-1330nm417nm
Magnesium Green™506nm531nm
Marina Blue®365nm460nm
mBanana540nm553nm
mCherry587nm610nm
mCitrine516nm529nm
mHoneydew487nm537nm
Midoriishi-Cyan (MiCy)472nm495nm
MidoriishiCyan/KusabiraOrange FRET472nm559nm
Mithramycin395nm535nm
MitoTracker® Deep Red 633/MeOH644nm665nm
MitoTracker® Green490nm516nm
MitoTracker® Orange551nm576nm
MitoTracker® Red578nm599nm
mKate2589nm634nm
mKeima Red440nm620nm
mKO (Kusabira Orange)548nm549nm
mOrange548nm562nm
mPlum590nm649nm
mRasberry598nm625nm
mRFP1584nm607nm
mStrawberry574nm596nm
mTangerine568nm585nm
mTFP1462nm492nm
mTurquoise434nm474nm
mWasabi493nm509nm
NBD465nm535nm
Nile Blue631nm660nm
Nile Red549nm628nm
NirFP604nm669nm
Oregon Green™ 488496nm524nm
Oregon Green™ 500503nm522nm
Oregon Green™ 514511nm530nm
Oregon Green™ 514506nm526nm
Pacific Blue?410nm455nm
PBF1334nm504nm
PhiYFP525nm537nm
PhiYFP-m525nm537nm
PKH26551nm567nm
POPO™-1, PO-PRO™-1434nm456nm
POPO™-3, PO-PRO™-3534nm572nm
Propidium Iodide536nm617nm
PS-CFP400nm468nm
PS-GFP490nm511nm
Pulsar™ 650460nm650nm
PyMPO415nm570nm
Pyrene345nm378nm
Pyronin Y555nm580nm
Qdot™ 525 ConjugateUV525nm
Qdot™ 565 ConjugateUV565nm
Qdot™ 585 ConjugateUV585nm
Qdot™ 605 ConjugateUV605nm
Qdot™ 655 ConjugateUV655nm
Qdot™ 705 ConjugateUV705nm
Qdot™ 800 ConjugateUV800nm
Quasar® 570548nm566nm
Quasar® 670647nm670nm
Quasar® 705690nm705nm
Quinacrine Mustard423nm503nm
ReAsH-CCPGCC592nm606nm
Red Fluorescent Protein (DsRed2)561nm585nm
Resorufin570nm585nm
RH 414500nm635nm
Rhod-2550nm571nm
Rhodamine 110496nm520nm
Rhodamine 123507nm529nm
Rhodamine 6G525nm555nm
Rhodamine B555nm580nm
Rhodamine Greenr™502nm527nm
Rhodamine Phalloidinr™542nm565nm
Rhodamine Redr™570nm590nm
ROX578nm604nm
R-phycocyanin618nm642nm
R-phycoerythrin (PE)565nm575nm
SBFI334nm525nm
SBFI/Na+ -free340nm505nm
SBFI/Na+ -saturated380nm505nm
SensiLight P-1550nm664nm
SensiLight P-3609nm661nm
Sirius355nm424nm
SITS337nm436nm
SNAFL®-1576nm635nm
SNAFL®-2525nm546nm
SNARF®-1575nm635nm
Sodium Greenr™507nm535nm
SpectrumAqua®433nm480nm
SpectrumBlue®400nm450nm
SpectrumFRed®655nm675nm
SpectrumGold®530nm555nm
SpectrumGreen®497nm524nm
SpectrumOrange®559nm588nm
SpectrumRed®587nm612nm
Sulforhodamine 101586nm605nm
SYBR® Green I498nm522nm
SYBR Gold495nm540nm
SYPRO Red550630
SYPRO Ruby280620
SYTO® 9/DNA483nm500nm
SYTO® 11508nm527nm
SYTO® 13488nm509nm
SYTO® 17621nm634nm
SYTO® 45452nm484nm
SYTO® 60652nm678nm
SYTOX® Blue445nm470nm
SYTOX® Green504nm523nm
SYTOX® Orange547nm570nm
TagBFP402nm457nm
TagCFP458nm480nm
TagGFP482nm505nm
TagRFP555nm583nm
TagYFP508nm524nm
TAMRA555nm580nm
tdTomato554nm581nm
TET521nm536nm
Tetramethylrhodamine (TRITC)555nm580nm
Texas Red®595nm620nm
Texas Red®-X595nm615nm
Thiadicarbocyanine651nm671nm
Topaz514nm529nm
TO-PRO™-5748nm768nm
TO-PRO™-3642nm661nm
TOTO™-1, TO-PRO™-1514nm533nm
TOTO™-3, TO-PRO™-3642nm660nm
TRITC555nm580nm
T-Sapphire398nm510nm
TurboFP602574nm602nm
TurboFP650592nm650nm
TurboGFP482nm502nm
TurboRFP553nm574nm
TurboYFP525nm538nm
Venus515nm528nm
WtGFP395nm508nm
WW 781605nm639nm
X-rhod-1/Ca2+580nm601nm
X-Rhodamine (XRITC)580nm605nm
YEP/TRITC FRET513nm580nm
YFP (Yellow Fluorescent Protein)513nm527nm
YFP/Cy3™ FRET513nm570nm
YFP/TRITC FRET513nm580nm
YOYO™-1, YO-PRO™-1491nm509nm
YOYO™-3, YO-PRO™-3612nm631nm


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